Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband®, Biotine Conjugate

Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband®, Biotine Conjugate

• Background: The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
• Description: Boster Bio Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband® catalog # PB9482. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Sodium/potassium-transporting ATPase subunit alpha-1;Na (+)/K (+) ATPase alpha-1 subunit;3.6.3.9;Sodium pump subunit alpha-1;ATP1A1;
• Gene Name: Sodium/potassium-transporting ATPase subunit alpha-1
• Gene ID: 476
• UniProt: P05023
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ATP1A1, different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.
• Clonality: Polyclonal
• Applications: IHC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
• References & Citations: 1. "Entrez Gene: ATP1A1 ATPase, Na+/K+transporting, alpha 1 polypeptide". 2. Dunbar LA, Caplan MJ (2001). "Ion pumps in polarized cells: sorting and regulation of the Na+, K+- and H+, K+-ATPases.". J. Biol. Chem. 276 (32): 29617–20. 3. Xie Z, Cai T (2004). "Na+-K+--ATPase-mediated signal transduction: from protein interaction to cellular function.". Mol. Interv. 3(3): 157–68.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 112896 MW
• Observed Molecular Weight: 100-113 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Mouse, Rat
  Immunohistochemistry, 2-5 μg/ml, Human, Mouse, Rat