Anti-HEF1/NEDD9 Antibody Picoband®, Cy3 Conjugate

Anti-HEF1/NEDD9 Antibody Picoband®, Cy3 Conjugate

• Background: Enhancer of filamentation 1 (EF1), also known as NEDD-9, is a protein that in humans is encoded by the NEDD9 gene. The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. It is mapped to 6p24.2. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. NEDD9 is also a highly relevant cancer gene that governs metastatic potential in human melanoma. What’s more, it has been speculated that NEDD9 may be an important linking element between extracellular signaling and regulation of the cytoskeleton.
• Description: Boster Bio Anti-HEF1/NEDD9 Antibody Picoband® catalog # PB9289. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Enhancer of filamentation 1;hEF1;CRK-associated substrate-related protein;CAS-L;CasL;Cas scaffolding protein family member 2;Neural precursor cell expressed developmentally down-regulated protein 9;NEDD-9;Renal carcinoma antigen NY-REN-12;p105;Enhancer of filamentation 1 p55;NEDD9;CASL, CASS2;
• Gene Name: Enhancer of filamentation 1
• Gene ID: 4739
• UniProt: Q14511
• Host: Rabbit
• Reactivity: Human,Monkey,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E.coli-derived human HEF1 recombinant protein (Position: K273-E421). Human HEF1 shares 83% amino acid (aa) sequence identity with mouse HEF1.
• Clonality: Polyclonal
• Applications: Flow Cytometry,IF,IHC,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
• References & Citations: 1. Law, S. F., Estojak, J., Wang, B., Mysliwiec, T., Kruh, G., Golemis, E. A. Human enhancer of filamentation 1, a novel p130(cas)-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae. Molec. Cell. Biol. 16: 3327-3337, 1996. 2. Gross, M. B. Personal Communication. Baltimore, Md. 9/4/2012.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 92861 MW
• Observed Molecular Weight: 93-105 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
  Immunohistochemistry, 2-5μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human