Anti-Integrin beta 4/ITGB4 Antibody Picoband®, Biotine Conjugate

Anti-Integrin beta 4/ITGB4 Antibody Picoband®, Biotine Conjugate

• Background: ITGB4 (Integrin, beta-4), also known as CD104 (Cluster of Differentiation 104), is a human gene. The gene encodes the integrin beta 4 subunits, a receptor for the laminins. This subunit tends to associate with alpha 6 subunits and is likely to play a pivotal role in the biology of invasive carcinoma. The ITGB4 gene is mapped on 17q25.1. Using expression profiling, Yang et al. found that ITGB4 was upregulated 6-fold by ZKSCAN3 in transfected human colon cancer cells compared with parental cells. They confirmed that ZKSCAN3 bound the promoter of ITGB4 in vitro and in vivo. ITGB4 knockdown by short hairpin RNA countered ZKSCAN3-augmented anchorage-independent colony formation in the colon cancer cell lines. The integrin beta-4 subunit is characterized by an unusually long cytoplasmic domain that harbors 4 fibronectin type III (FNIII) repeats, residing in 2 pairs separated by a connecting segment. Vidal et al. found compound heterozygosity for mutations in the ITGB4 gene in an infant with junctional epidermolysis bullosa associated with pyloric atresia.
• Description: Boster Bio Anti-Integrin beta 4/ITGB4 Antibody Picoband® catalog # PB9007. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Integrin beta-4;GP150;CD104;ITGB4;
• Gene Name: Integrin beta-4
• UniProt: P16144
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E.coli-derived human Integrin beta 4 recombinant protein (Position: N28-A266). Human Integrin beta 4 shares 88% and 86% amino acid (aa) sequences identity with mouse and rat Integrin beta 4, respectively.
• Clonality: Polyclonal
• Applications: Flow Cytometry,IF,IHC,IHC-F,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. .
• References & Citations: 1. Carmi, R., Sofer, S., Karplus, M., Ben-Yakar, Y., Mahler, D., Zirkin, H., Bar-Ziv, J. Aplasia cutis congenita in two sibs discordant for pyloric atresia. Am. J. Med. Genet. 11: 319-328, 1982. 2. Dajee, M., Lazarov, M., Zhang, J. Y., Cai, T., Green, C. L., Russell, A. J., Marinkovich, M. P., Tao, S., Lin, Q., Kubo, Y., Khavari, P. A. NF-kappa-B blockade and oncogenic Ras trigger invasive human epidermal neoplasia. Nature 421: 639-643, 2003. 3. Geerts, D., Fontao, L., Nievers, M. G., Schaapveld, R. Q. J., Purkis, P. E., Wheeler, G. N., Lane, E. B., Leigh, I.M., Sonnenberg, A.Binding of integrin alpha-6-beta-4 to plectin prevents plectin association with F-actin but does not interfere with intermediate filament binding. J. Cell Biol. 147: 417-434, 1999.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 202167 MW
• Observed Molecular Weight: 210 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
  Immunofluorescence, 2μg/ml, Human
  Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
  Immunocytochemistry, 0.5-1μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human