Anti-Transcription factor E2F1 E2F1 Antibody Picoband®, Biotine Conjugate

Anti-Transcription factor E2F1 E2F1 Antibody Picoband®, Biotine Conjugate

• Background: Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family of transcription factors appears to play a critical role in the transcription of certain genes required for cell cycle progression. E2F1, the first cloned member of this family, is regulated during the cell cycle at the mRNA level by changes in transcription of the E2F1 gene and at the protein level by complex formation with proteins such as the retinoblastoma gene product (pRB), cyclin A and DP1. E2F1 can override a pRB-induced G1/S block and can behave as an oncogene in certain cells. E2F1 was cloned and was found to contain seven exons. Fluorescence in situ hybridization localized E2F1 to chromosome 20q11. The E2F1 transcription factor can promote proliferation or apoptosis when activated, and is a key downstream target of the retinoblastoma tumour suppressor protein (pRB).
• Description: Boster Bio Anti-Transcription factor E2F1 E2F1 Antibody catalog # PA1560. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Transcription factor E2F1;E2F-1;PBR3;Retinoblastoma-associated protein 1;RBAP-1;Retinoblastoma-binding protein 3;RBBP-3;pRB-binding protein E2F-1;E2F1;RBBP3;
• Gene Name: Transcription factor E2F1
• UniProt: Q01094
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human E2F1, identical to the related mouse and rat sequences.
• Clonality: Polyclonal
• Applications: WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). .
• References & Citations: 1. Neuman E, Sellers WR, McNeil JA, Lawrence JB, Kaelin WG Jr (Dec 1996). "Structure and partial genomic sequence of the human E2F1 gene". Gene 173 (2): 163–9. 2. Morris, E. J., Ji, J.-Y., Yang, F., DiStefano, L., Herr, A., Moon, N.-S., Kwon, E.-J., Haigis, K. M., Naar, A. M., Dyson, N. J. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. Nature 455: 552-556, 2008. 3. Neuman, E., Sellers, W. R., McNeil, J. A., Lawrence, J. B., Kaelin, W. G., Jr. Structure and partial genomic sequence of the human E2F1 gene. Gene 173: 163-169, 1996.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 46920 MW
• Observed Molecular Weight: 60 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human , Mouse, Rat