Human CXCL16 PicoKine® Quick ELISA Kit

Human CXCL16 PicoKine® Quick ELISA Kit

• Background: Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16 (CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.
• Description: Human CXCL16 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CXCL16 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: C-X-C motif chemokine 16;Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein;SR-PSOX;Small-inducible cytokine B16;Transmembrane chemokine CXCL16;CXCL16;SCYB16, SRPSOX;UNQ2759/PRO6714;
• Gene Name: C-X-C motif chemokine 16
• Gene ID: 58191
• UniProt: Q9H2A7
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: N49-P137
• Applications: ELISA
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 93.7 pg/ml - 6,000 pg/ml
• Sensitivity: <10 pg/ml
• Storage Conditions: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
• Specificity: Natural and recombinant human CXCL16
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	122	112	116	125	118	5.06	4.2%
  Sample 2	725	748	679	765	729	32.29	4.4%
  Sample 3	2351	2384	2122	2446	2325	122.47	5.2%

  *number of samples for each test n=16.
• : heparin or EDTA
• Calculated Molecular Weight: 27579 MW