Anti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband®, Biotine Conjugate

Anti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband®, Biotine Conjugate

• Background: The mitochondrial dicarboxylate carrier (DIC) is an integral membrane protein encoded by the SLC25A10 gene in humans that catalyzes the transport of dicarboxylates such as malonate, malate, and succinate across the inner mitochondrial membrane in exchange for phosphate, sulfate, and thiosulfate by a simultaneous antiport mechanism, thus supplying substrates for the Krebs cycle, gluconeogenesis, urea synthesis, fatty acid synthesis, and sulfur metabolism. This gene encodes a member of a family of proteins that translocate small metabolites across the mitochondrial membrane. The encoded protein exchanges dicarboxylates, such as malate and succinate, for phosphate, sulfate, and other small molecules, thereby providing substrates for metabolic processes including the Krebs cycle and fatty acid synthesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Description: Boster Bio Anti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® catalog # A10727-3. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: RNA-binding protein Nova-2; Astrocytic NOVA1-like RNA-binding protein; Neuro-oncological ventral antigen 2; NOVA2; ANOVA; NOVA3
• Gene Name: NOVA alternative splicing regulator 2
• Gene ID: 1468
• UniProt: Q9UBX3
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human Mitochondrial dicarboxylate carrier/SLC25A10 recombinant protein (Position: Q37-Q276).
• Clonality: Polyclonal
• Applications: WB,IHC,IF,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: May regulate RNA splicing or metabolism in a specific subset of developing neurons. Binds single strand RNA.
• References & Citations: 1. Fiermonte, G., Dolce, V., Arrigoni, R., Runswick, M. J., Walker, J. E., Palmieri, F. Organization and sequence of the gene for the human mitochondrial dicarboxylate carrier: evolution of the carrier family. Biochem. J. 344: 953-960, 1999. 2. Mizuarai, S., Miki, S., Araki, H., Takahashi, K., Kotani, H. Identification of dicarboxylate carrier Slc25a10 as malate transporter in de novo fatty acid synthesis. J. Biol. Chem. 280: 32434-32441, 2005. 3. Pannone, E., Fiermonte, G., Dolce, V., Rocchi, M., Palmieri, F. Assignment of the human dicarboxylate carrier gene (DIC) to chromosome 17 band 17q25.3. Cytogenet. Cell Genet. 83: 238-239, 1998.
• Storage Conditions: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Calculated Molecular Weight: 29370 MW
• Observed Molecular Weight: 29-31 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.25-0.5 μg/ml, Human, Rat
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
  Immunofluorescence, 5 μg/ml, Human
  ELISA, 0.1-0.5 μg/ml, -