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RAP80 Antibody

CAT: 0013-GTR17697947Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17697947Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to UIMC1
Product Name Alternative
Anti UIMC1 antibody, anti ubiquitin interaction motif containing 1 antibody, anti RAP80 antibody, anti X2HRIP110 antibody, anti OTTHUMP00000161441 antibody, anti receptor associated protein 80 antibody, anti retinoid x receptor interacting protein antibody
Reactivity
Human
Target
RAP80
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
TRRQKEAKTKSDSG
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml
Molecular Weight
79.7
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 60kDa band in Human Heart and Spleen lysates and in lysates of cell lines A431, HeLa and HepG2 after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.7kDa according to NP_057374.3. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
51720

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