Anti-NDUFS8 Antibody Picoband®, HRP Conjugate

Anti-NDUFS8 Antibody Picoband®, HRP Conjugate

• Background: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial also known as NADH-ubiquinone oxidoreductase 23 kDa subunit, Complex I-23kD (CI-23kD), or TYKY subunit is an enzyme that in humans is encoded by the NDUFS8 gene. This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome.
• Description: Boster Bio Anti-NDUFS8 Antibody Picoband® catalog # A08275-2. Tested in ELISA, Flow Cytometry, IHC, IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Neuropeptides B/W receptor type 1; G-protein coupled receptor 7; NPBWR1; GPR7
• Gene Name: neuropeptides B and W receptor 1
• Gene ID: 4728
• UniProt: O00217
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human NDUFS8 recombinant protein (Position: M1-R210).
• Clonality: Polyclonal
• Applications: WB,IHC,Flow Cytometry,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals. Has a higher affinity for neuropeptide B.
• References & Citations: 1. de Sury, R., Martinez, P., Procaccio, V., Lunardi, J., Issartel, J.-P. Genomic structure of the human NDUFS8 gene coding for the iron-sulfur TYKY subunit of the mitochondrial NADH:ubiquinone oxidoreductase. Gene 215: 1-10, 1998. 2. Dunbar, D. R., Shibasaki, Y., Dobbie, L., Andersson, B., Brookes, A. J. In situ hybridisation mapping of genomic clones for five human respiratory chain complex I genes. Cytogenet. Cell Genet. 78: 21-24, 1997. 3. Emahazion, T., Brookes, A. J. Mapping of the NDUFA2, NDUFA6, NDUFA7, NDUFB8, and NDUFS8 electron transport chain genes by intron based radiation hybrid mapping. Cytogenet. Cell Genet. 82: 114 only, 1998.
• Storage Conditions: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Observed Molecular Weight: 23 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
  Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
  ELISA, 0.1-0.5 μg/ml, -