Anti-U1-C/SNRPC Antibody Picoband®, Biotine Conjugate

Anti-U1-C/SNRPC Antibody Picoband®, Biotine Conjugate

• Background: U1 small nuclear ribonucleoprotein C is a protein that in humans is encoded by the SNRPC gene. This gene encodes one of the specific protein components of the U1 small nuclear ribonucleoprotein (snRNP) particle required for the formation of the spliceosome. The encoded protein participates in the processing of nuclear precursor messenger RNA splicing. snRNP particles are attacked by autoantibodies frequently produced by patients with connective tissue diseases. The genome contains several pseudogenes of this functional gene. Alternative splicing results in a non-coding transcript variant.
• Description: Boster Bio Anti-U1-C/SNRPC Antibody Picoband® catalog # A07728. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Protein SOX-15; Protein SOX-12; Protein SOX-20; SOX15; SOX12; SOX20; SOX26; SOX27
• Gene Name: SRY-box 15
• Gene ID: 6631
• UniProt: P09234
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human U1-C/SNRPC recombinant protein (Position: M1-R159).
• Clonality: Polyclonal
• Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Binds to the 5'-AACAAT-3' sequence.
• References & Citations: 1. Du, H., Rosbash, M. The U1 snRNP protein U1C recognizes the 5-prime splice site in the absence of base pairing. Nature 419: 86-90, 2002. 2. Nelissen, R. L. H., Klein Gunnewiek, J. M. T., Lambermon, M. H. L., Van Venrooij, W. J. Cloning and characterization of two processed pseudogenes and the cDNA for the murine U1 snRNP-specific protein C. Gene 184: 273-278, 1997. 3. Sillekens, P. T. G., Beijer, R. P., Habets, W. J., van Venrooij, W. J. Human U1 snRNP-specific C protein: complete cDNA and protein sequences and identification of a multigene family in mammals. Nucleic Acids Res. 16: 8307-8321, 1988.
• Storage Conditions: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Calculated Molecular Weight: 49229 MW
• Observed Molecular Weight: 17 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.25-0.5 μg/ml, Human, Mouse
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -