Anti-Ribophorin I/RPN1 Antibody Picoband®, Biotine Conjugate

Anti-Ribophorin I/RPN1 Antibody Picoband®, Biotine Conjugate

• Background: Dolichyl-diphosphooligosaccharide—protein glycosyltransferase subunit 1 is an enzyme that in humans is encoded by the RPN1 gene. This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
• Description: Boster Bio Anti-Ribophorin I/RPN1 Antibody Picoband® catalog # A05063-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Centriolar coiled-coil protein of 110 kDa; Centrosomal protein of 110 kDa; CP110; Cep110; CCP110; CEP110; CP110; KIAA0419
• Gene Name: centriolar coiled-coil protein 110
• Gene ID: 6184
• UniProt: P04843
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human Ribophorin I/RPN1 recombinant protein (Position: A24-Q148).
• Clonality: Polyclonal
• Applications: WB,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation (PubMed:17719545 PubMed:17681131, PubMed:23486064). Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation (By similarity). Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2 (PubMed:16760425).
• References & Citations: 1. Barton, D. E., Crimaudo, C., Hortsch, M., Francke, U. The genes for ribophorins I and II are on human chromosomes 3q and 20 and mouse chromosomes 6 and 12, respectively. (Abstract) Cytogenet. Cell Genet. 46: 577 only, 1987. 2. Crimaudo, C., Hortsch, M., Gausepohl, H., Meyer, D. I. Human ribophorins I and II: the primary structure and membrane topology of two highly conserved round endoplasmic reticulum-specific glycoproteins. EMBO J. 6: 75-82, 1987. 3. Dumax-Vorzet, A., Roboti, P., High, S. OST4 is a subunit of the mammalian oligosaccharyltransferase required for efficient N-glycosylation. J. Cell Sci. 126: 2595-2606, 2013.
• Storage Conditions: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Calculated Molecular Weight: 39125 MW
• Observed Molecular Weight: 68 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.25-0.5 μg/ml, Human
  ELISA, 0.1-0.5 μg/ml, -