Anti-NEDD8 Antibody Picoband®, Biotine Conjugate

Anti-NEDD8 Antibody Picoband®, Biotine Conjugate

• Background: NEDD8 is a protein that in humans is encoded by the NEDD8 gene. Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The most substrates of NEDD8 modification are the Cullin subunits of Cullin-based E3 ubiquitin ligases, which are active only when neddylated. Their NEDDylation is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.
• Description: Boster Bio Anti-NEDD8 Antibody Picoband® catalog # A00547. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: NEDD8;Neddylin;Neural precursor cell expressed developmentally down-regulated protein 8;NEDD-8;Ubiquitin-like protein Nedd8;NEDD8;
• Gene Name: NEDD8
• Gene ID: 4738
• UniProt: Q15843
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NEDD8, identical to the related mouse and rat sequences.
• Clonality: Polyclonal
• Applications: Flow Cytometry,IF,IHC,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. .
• References & Citations: 1. Bohnsack, R. N., Haas, A. L. Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. J. Biol. Chem. 278: 26823-26830, 2003. 2. Cope, G. A., Suh, G. S. B., Aravind, L., Schwarz, S. E., Zipursky, S. L., Koonin, E. V., Deshaies, R. J. Role of predicted metalloprotease motif of Jab1/Csn5 in cleavage of Nedd8 from Cul1. Science 298: 608-611, 2002. 3. Cui, J., Yao, Q., Li, S., Ding, X., Lu, Q., Mao, H., Liu, L., Zheng, N., Chen, S., Shao, F. Glutamine deamidation and dysfunction of ubiquitin/NEDD8 induced by a bacterial effector family. Science 329: 1215-1218, 2010.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 9072 MW
• Observed Molecular Weight: 12 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
  Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human