Anti-Integrin Alpha 4/Itga4 Antibody Picoband®

Anti-Integrin Alpha 4/Itga4 Antibody Picoband®

• Background: CD49d is an integrin alpha subunit. It makes up half of the α4β1 lymphocyte homing receptor. The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants.
• Description: Boster Bio Anti-Integrin Alpha 4/Itga4 Antibody Picoband® catalog # A00277-2. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
• Gene Name: Serine/threonine-protein kinase AtPK1/AtPK6
• Gene ID: 16401
• UniProt: Q00651
• Host: Rabbit
• Reactivity: Mouse,Rat
• Cross Reactivity: No cross reactivity with other proteins.
• Immunogen: E.coli-derived mouse Integrin Alpha 4/Itga4 recombinant protein (Position: Y41-D963). Mouse Itga4 shares 85.2% amino acid (aa) sequence identity with human Itga4.
• Clonality: Polyclonal
• Applications: WB,Flow Cytometry,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.
• References & Citations: 1. Cunningham, S. A., Rodriguez, J. M., Arrate, M. P., Tran, T. M., Brock, T. A. JAM2 interacts with alpha-4/beta-1: facilitation by JAM3. J. Biol. Chem. 277: 27589-27592, 2002. 2. Fernandez-Ruiz, E., Pardo-Manuel de Villena, F., Rubio, M. A., Corbi, A. L., Rodriguez de Cordoba, S., Sanchez-Madrid, F. Mapping of the human VLA-alpha-4 gene to chromosome 2q31-q32. Europ. J. Immun. 22: 587-590, 1992. 3. Garmy-Susini, B., Jin, H., Zhu, Y., Sung, R.-J., Hwang, R., Varner, J. Integrin alpha-4-beta-1--VCAM-1--mediated adhesion between endothelial and mural cells is required for blood vessel maturation. J. Clin. Invest. 115: 1542-1551, 2005.
• Storage Conditions: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Calculated Molecular Weight: 52588 MW
• Observed Molecular Weight: 150-180 kDa
• Fragment: IgG
• Applications Notes: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
  ELISA, 0.1-0.5 μg/ml, -