Products for Research Use Only

SAFB2 Antibody

CAT: 0013-GTR17697817Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17697817Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to SAFB2
Product Name Alternative
Anti SAFB2 antibody, anti scaffold attachment factor B2 antibody, anti KIAA0138 antibody
Target
SAFB2
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
GEHNQRLEEHQARA
Field of Research
Epigenetics
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1 μg/ml
Molecular Weight
107
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 75kDa and 60kDa in lysates of HeLa cell lines after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 107kDa according to NP_055464.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Human
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
9667