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ATG4D Antibody

CAT: 0013-GTR17697804Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17697804Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to ATG4D
Product Name Alternative
Anti ATG4D antibody, anti ATG4 autophagy related 4 homolog D (S. cerevisiae) antibody, anti APG4-D antibody, anti APG4D antibody, anti AUTL4 antibody, anti APG4 autophagy 4 homolog D antibody, anti AUT-like 4, cysteine endopeptidase antibody, anti autophagin-4 antibody, anti autophagy-related cysteine endopeptidase 4 antibody, anti cysteine protease involved in autophagy antibody
Reactivity
Human
Target
ATG4D
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
CSQLAQPTLRLPRT
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1 μg/ml, IHC-P: 2.5 μg/ml
Molecular Weight
52.9
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx. 26kDa band in Human Skeletal Muscle lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kDa according to NP_116274.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
84971

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