Recombinant Mouse Prostatic Acid Phosphatase Protein(Fc Tag)

Recombinant Mouse Prostatic Acid Phosphatase Protein(Fc Tag)

• Background: ACPP (Acid phosphatase, prostate, also PAP and ACP3) is a 48-52 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP. This generates adenosine which acts as a strong analgesic agent. Mature Human ACPP is 354 amino acids (aa) in length (aa 33-386). It contains one histidine phosphatase domain (aa 34-332), plus a nucleophile acceptor site at His44, and a proton donor site at Asp290. There are two potential alternative splice variants. One shows a deletion of aa 153-185, while another is transmembrane (previously called TMPase) and shows a 38 aa substitution for the C-terminal seven amino acids. Over aa 33-379, Human ACPP shares 84% aa identity with an Mouse ACPP.
• Abbreviation: Prostatic Acid Phosphatase/ACPP
• Synonyms: 5'-NT, 5'-nucleotidase, Acid phosphatase prostate, ACP 3, ACP3, acpP, Ecto-5'-nucleotidase, PAP, PPAP, Prostatic acid phosphatase, Prostatic acid phosphotase, Thiamine monophosphatase, TMPase
• CAS Number: 9000-83-3
• UniProt: Q8CE08
• Accession Number: Q8CE08
• Host: Mammalian
• Origin Species: Mouse
• Tag: C-Fc
• Sequence: Met1-Asn381aa
• Field of Research: Tags & Cell Markers;Cell Biology;Cancer
• Purity: > 90% as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
• Molecular Weight: 80 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.