Products for Research Use Only

SPHK1 Antibody

CAT: 0013-GTR17697614Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17697614Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to SPHK1
Product Name Alternative
Anti SPHK1 antibody, anti sphingosine kinase 1 antibody, anti SPHK antibody, anti SK1 antibody, anti SKase antibody
Reactivity
Human
Target
SPHK1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DVDLESEKYRRLGE
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 2-3 μg/ml
Molecular Weight
43.9; 51.1; 42.5
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2) . Recommended concentration: 2-3 μg/ml.Experiment Notes: Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected, see Igarashi N. et al. 2003: Figure 2B), PMID: 12954646.
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, IP, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
8877

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