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Human F10 protein

CAT: 0013-GTR18905564-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18905564-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human Factor X Protein is produced by mammalian expression system and the target gene encoding Asn32-Lys488 is expressed with a Fc tag at the C-terminus.
Product Name Alternative
Coagulation factor X; Stuart factor; Stuart-Prower factor
UniProt
P00742
Reactivity
Human
Source
Human cells
Field of Research
Cardiovascular Research
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Form
Lyophilized
Storage Conditions
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μm filtered solution of 20mM MES, 150mM NaCl, 0.2mM CaCl2, pH 5.5.
AA Sequence
NNILARVTRA NSFLEEMKKG HLERECMEET CSYEEAREVF EDSDKTNEFW NKYKDGDQCE TSPCQNQGKC KDGLGEYTCT CLEGFEGKNC ELFTRKLCSL DNGDCDQFCH EEQNSVVCSC ARGYTLADNG KACIPTGPYP CGKQTLERRK RSVAQATSSS GEAPDSITWK PYDAADLDPT ENPFDLLDFN QTQPERGDNN LTRIVGGQEC KDGECPWQAL LINEENEGFC GGTILSEFYI LTAAHCLYQA KRFKVRVGDR NTEQEEGGEA VHEVEVVIKH NRFTKETYDF DIAVLRLKTP ITFRMNVAPA CLPERDWAES TLMTQKTGIV SGFGRTHEKG RQSTRLKMLE VPYVDRNSCK LSSSFIITQN MFCAGYDTKQ EDACQGDSGG PHVTRFKDTY FVTGIVSWGE GCARKGKYGI YTKVTAFLKW IDRSMKTRGL PKAKSHAPEV ITSSPLKDIE GRMDEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK
Entrez
2159

UniProtKB · P00742

Coagulation factor X

FA10_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P00742
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
F10
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.21.6
Processing
Precursor
Secondary accessions
Q14340
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Blood coagulationHemostasis

Ligand

Calcium

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acidGlycoproteinHydroxylationZymogen

Disease

Disease variant

Domain

EGF-like domainRepeatSignal

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

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