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Human UBE2A protein

CAT: 0013-GTR18906177-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18906177-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant human UBE2A protein is produced by our E.coli expression system and the target gene encoding Met1-Cys152 is expressed with a GST, 6His tag at the N-terminus.
Product Name Alternative
RAD6A; Ubiquitin-conjugating enzyme E2 A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A
UniProt
P49459
Reactivity
Human
Source
E. coli
Field of Research
Epigenetics & Chromatin
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Form
Liquid
Storage Conditions
Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
AA Sequence
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGDDDDKSPM GSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC
Entrez
7319

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