GENIUS™Nuclease

GENIUS™Nuclease

• Description: GENIUS™Nuclease is a recombinant form of Serratia marcescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-5 bases in length.
• Specifications: BenzNuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. BenzNuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length.
• Host: E. coli
• Target: Nuclease
• Conjugation: Unconjugated
• Tag: Native
• Source: Serratia marcescens
• Stability: Avoid repeated freeze-thaw cycles. This product is stable after storage at: In lyophilized state for 1 year (-20oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Format: Powder
• Buffer: 20mM Tris, 20mM NaCl, 2mM MgCl2, pH8.0
• Molecular Weight: 26.8 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C