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MSH4 (internal) Antibody

CAT: 0013-GTR17697531Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17697531Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to MSH4
Product Name Alternative
Anti MSH4 antibody, anti mutS homolog 4 (E. coli) antibody, anti RP11-262K24.2 antibody, anti mutS homolog 4 antibody
Target
MSH4 (internal)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
ETNSSTFMKEMKE
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.1 μg/ml
Molecular Weight
105
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx 60kDa and 35kDa in Human Testis, Ovary and Uterus lysates and in Mouse and Rat Testis lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 105kDa according to NP_002431.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Human, Mouse
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
4438

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