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Recombinant Human Mucin-16 (MUC16), partial (Active)

CAT: 0013-GTR18894212-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18894212-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This Recombinant Human Mucin-16 (MUC16), partial (Active) spans the amino acid sequence from region 12660-12923aa. Purity: Greater than 95% as determined by SDS-PAGE.
UniProt
Q8WXI7
Expression Region
12660-12923aa
Tag
C-terminal 6xHis-tagged
Source
Mammalian cell
Origin
Homo sapiens (Human)
Field of Research
Cancer Biology
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE.
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized MUC16 at 10 μg/ml can bind MSLN, the EC50 is 460.7-662.2 ng/ml.
Form
Lyophilized powder
Molecular Weight
30.5 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
AA Sequence
GFTHWIPVPTSSTPGTSTVDLGSGTPSSLPSPTTAGPLLVPFTLNFTITNLKYEEDMHCPGSRKFNTTERVLQSLLGPMFKNTSVGPLYSGCRLTLLRSEKDGAATGVDAICTHRLDPKSPGVDREQLYWELSQLTNGIKELGPYTLDRNSLYVNGFTHQTSAPNTSTPGTSTVDLGTSGTPSSLPSPTSAGPLLVPFTLNFTITNLQYEEDMHHPGSRKFNTTERVLQGLLGPMFKNTSVGLLYSGCRLTLLRPEKNGAATGM

UniProtKB · Q8WXI7

Mucin-16

MUC16_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8WXI7
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MUC16
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MUC-16
EC number
—
Processing
—
Secondary accessions
B5ME49, Q6ZQW5, Q96RK2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

RepeatTransmembraneTransmembrane helix

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