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NKD2 Antibody

CAT: 0013-GTR17697410Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17697410Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to NKD2
Product Name Alternative
Anti NKD2 antibody, anti naked cuticle homolog 2 (Drosophila) antibody, anti Dvl-binding protein NKD2 antibody, anti naked cuticle homolog 2 antibody, anti naked cuticle-2 antibody
Target
NKD2
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
HKRYRQKGREGHS
Field of Research
Stem Cells
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.1 μg/ml
Molecular Weight
50.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 80kDa, 38kDa and 26kDa in Mouse Brain lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 51.5kDa according to Mouse NP_082462.2 and of 50.1kDa according to Human NP_149111.1. All the detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Human, Mouse, Rat
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
85409

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