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E. coli RTCB protein

CAT: 0013-GTR18882981-03Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18882981-03Size:1 mg
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Description
This E. coli RTCB protein spans the amino acid sequence from region 1-408aa. Purity: Greater than 90% as determined by SDS-PAGE.
Product Name Alternative
RtcB, yhgL, b3421, JW3384, RNA-splicing ligase RtcB, EC 6.5.1.8, 3'-phosphate/5'-hydroxy nucleic acid ligase
UniProt
P46850
Expression Region
1-408aa
Tag
N-terminal 6xHis-SUMO-tagged
Source
E.coli
Origin
Escherichia coli (strain K12)
Field of Research
Infectious Disease & Virology
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Molecular Weight
61.2 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
Tag Info: N-terminal 6xHis-SUMO-taggedExpression Region: 1-408aaSequence Info: Extracellular Domain
Preservative
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
AA Sequence
MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIAVMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQAIETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHLGTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRDLAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVEVIYTLRQVVCVKG

UniProtKB · P46850

RNA-splicing ligase RtcB

RTCB_ECOLI · Escherichia coli (strain K12)

View on UniProt ↗
Primary accession
P46850
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
rtcB
Protein existence
1: Evidence at protein level
Organism
Escherichia coli (strain K12)
Taxonomy ID
83333
Alternative names
—
EC number
6.5.1.8
Processing
—
Secondary accessions
P76690, Q2M785
Protein keywords

Ligand

GTP-bindingManganeseMetal-bindingNucleotide-binding

Molecular function

Ligase

Technical term

Reference proteome

Biological process

RNA repair