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Monkey IL3 protein (Active)

CAT: 0013-GTR18881528-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18881528-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This Monkey IL3 protein (Active) spans the amino acid sequence from region 20-143aa. Purity: > 98% as determined by SDS-PAGE and HPLC.
Product Name Alternative
IL-3, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor
UniProt
P25140
Expression Region
20-143aa
Tag
Tag-Free
Source
E.Coli
Origin
Macaca mulatta (Rhesus macaque)
Field of Research
Immunology & Inflammation
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
> 98% as determined by SDS-PAGE and HPLC.
Bioactivity
Assay #1: Fully biologically active when compared to standard. The ED50 as determined by the dose- dependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 x 10 ^ 5 IU/mg. Assay #2: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10 ^ 7 IU/mg.
Form
Lyophilized powder
Molecular Weight
14.0 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
Lyophilized from a 0.2 μm filtered PBS, pH 7.4, 5 % trehalose
AA Sequence
APMTQTTSLKTSWAKCSNMIDEIITHLNQPPLPSPDFNNLNEEDQTILVEKNLRRSNLEAFSKAVKSLQNASAIESILKNLPPCLPMATAAPTRPPIRITNGDRNDFRRKLKFYLKTLENEQAQ

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