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Rat IL-4 ELISA Kit

CAT: 0013-GTR18879013-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18879013-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Rat IL-4 ELISA Kit employs a two-site sandwich ELISA to quantitate Rat IL-4.
Product Name Alternative
IL 4; IL4; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1
Reactivity
Rat
Field of Research
Immunology & Inflammation
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
1.0 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Rat IL-4 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-4 in samples. An antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
1.56 pg/mL-100 pg/mL

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