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Mouse VEGF ELISA Kit

CAT: 0013-GTR18878957-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18878957-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse VEGF-A.
Product Name Alternative
VEGFA; RP1-261G23.1; MGC70609; MVCD1; VEGF; VEGF-A; VPF; vascular endothelial growth factor isoform VEGF165; vascular permeability factor
Reactivity
Mouse
Field of Research
Cardiovascular Research; Cell Biology
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
8 pg/ml
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Mouse VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF-A in samples. An antibody specific for VEGF-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF-A is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
15.6 pg/ml-1000 pg/ml

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