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Human IL-5 ELISA Kit

CAT: 0013-GTR18878580-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18878580-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human IL-5 ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-5.
Product Name Alternative
IL 5; IL5; IL-5; IL-5T-cell replacing factor; interleukin-5; TRF; TRFB cell differentiation factor I
Reactivity
Human
Field of Research
Immunology & Inflammation
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
4 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Human IL-5 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-5 in samples. An antibody specific for IL-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-5 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
7.8 pg/mL-500 pg/mL

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