Products for Research Use Only

Human HGF ELISA Kit

CAT: 0013-GTR18879101-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18879101-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human HGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human HGF.
Product Name Alternative
DFNB39; F TCF; Fibroblast derived tumor cytotoxic factor; Hepatocyte growth factor; Hepatopoietin A; Hepatopoietin-A; Hgf; HGFB; HPTA
Reactivity
Human
Field of Research
Cancer Biology
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
60 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Human HGF ELISA Kit employs a two-site sandwich ELISA to quantitate HGF in samples. An antibody specific for HGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGF is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
125 pg/mL-8000 pg/mL

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