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Human bFGF ELISA Kit

CAT: 0013-GTR18878768-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18878768-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human bFGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human bFGF.
Product Name Alternative
Basic fibroblast growth factor; BFGF; FGF 2; FGF B; FGF2; FGF2 basic; FGFB; Fibroblast growth factor 2 (basic) ; HBGF 2; HBGF2; HBGH 2; Heparin binding growth; factor 2; Heparin binding growth factor 2 precursor; OTTMUSP00000046776; Prostatropin
Reactivity
Human
Field of Research
Cardiovascular Research
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
8 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Human bFGF ELISA Kit employs a two-site sandwich ELISA to quantitate bFGF in samples. An antibody specific for bFGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any bFGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for bFGF is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of bFGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
15.6 pg/mL-1000 pg/mL

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