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Human EGF ELISA Kit

CAT: 0013-GTR18878657-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18878657-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human EGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human EGF.
Product Name Alternative
Beta urogastrone; Epidermal growth factor; HOMG4; OTTHUMP00000219721; OTTHUMP00000219722; Pro epidermal growth factor; URG; Urogastrone
Reactivity
Human
Field of Research
Cancer Biology
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
2 pg/Ml
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Human EGF ELISA Kit employs a two-site sandwich ELISA to quantitate EGF in samples. An antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGF is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
3.9 pg/mL-250 pg/mL

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