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ENOX2/APK1 Antibody

CAT: 0013-GTR17697315Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17697315Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal to APK1
Product Name Alternative
Anti ecto-NOX disulfide-thiol exchanger 2 antibody, anti RP5-875H3.1 antibody, anti APK1 antibody, anti COVA1 antibody, anti tNOX antibody, anti APK1 antigen antibody, anti cytosolic ovarian carcinoma antigen 1 antibody
Reactivity
Human
Target
ENOX2 / APK1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
EKLKDDKLQVEK
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, IHC-P: 2-4μg/ml
Molecular Weight
66.6; 70.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This antibody is expected to recognise both reported isoforms (NP_006366.2; NP_872114.1) .ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Kidney shows cytoplasm staining preferentially in PC. Recommended concentration, 2-4μg/ml.WB: Preliminary experiments gave bands at approx 60kDa and 37kDa in Human Heart lysates after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 66.6kDa and 70.1kDa according to NP_006366.2 and NP_872114.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
10495

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