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Human High Sensitivity IFNα (Interferon Alpha) ELISA Kit

CAT: 0013-GTR18875371-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18875371-01Size:48 Tests
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Description
Human High Sensitivity IFNα (Interferon Alpha) ELISA Kit
Product Name Alternative
Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D (LeIF D), IFNA1, IFNA13
UniProt
P01562
Reactivity
Human
Target
IFNα
Field of Research
Infectious Disease & Virology; Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sensitivity
0.15 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFNα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFNα. You can calculate the concentration of Human IFNα in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.78-50pg/mL

UniProtKB · P0DY56

Interferon alpha-1

IFNA1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P0DY56
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IFNA1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IFN-alpha-1
EC number
—
Processing
Precursor
Secondary accessions
D4Q9M8, P01562, Q14605, Q2M1L8, Q52LB8, Q5VYQ2, Q7M4Q1, Q8WZ68, Q9UMJ3
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Biological process

Antiviral defense

Molecular function

Cytokine

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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