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Mouse MFN1 (Mitofusin 1) Microsample ELISA Kit

CAT: 0013-GTR18873579-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873579-01Size:48 Tests
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Description
Mouse MFN1 (Mitofusin 1) Microsample ELISA Kit
Product Name Alternative
Hfzo1; Hfzo2; Transmembrane GTPase MFN1; Fzo homolog
UniProt
Q811U4
Reactivity
Mouse
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.071 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MFN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MFN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MFN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MFN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · Q811U4

Mitofusin-1

MFN1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q811U4
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Mfn1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.6.5.-
Processing
—
Secondary accessions
Q3URC4, Q811D5, Q8CEY6, Q99M10, Q9D395
Protein keywords

Technical term

3D-structureReference proteome

Domain

Coiled coilTransmembraneTransmembrane helix

Molecular function

Developmental proteinHydrolase

Ligand

GTP-bindingNucleotide-binding

Cellular component

MembraneMitochondrionMitochondrion outer membrane

PTM

Ubl conjugation

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