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Human ECP (Eosinophil Cationic Protein) Microsample ELISA Kit

CAT: 0013-GTR18873564-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873564-01Size:48 Tests
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Description
Human ECP (Eosinophil Cationic Protein) Microsample ELISA Kit
Product Name Alternative
Ribonuclease 3; RNase 3
UniProt
P12724
Reactivity
Human
Field of Research
Cancer Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
1.54 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ECP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ECP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ECP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ECP in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
3.13-200 ng/mL

UniProtKB · P12724

Eosinophil cationic protein

ECP_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P12724
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
RNASE3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
ECP
EC number
3.1.27.-
Processing
Precursor
Secondary accessions
Q4VBC1, Q8WTP7, Q8WZ62, Q9GZN9
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Molecular function

AntibioticAntimicrobialEndonucleaseHydrolaseNuclease

PTM

Disulfide bondGlycoproteinNitration

Cellular component

Secreted

Domain

Signal

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