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Mouse GS (Gelsolin) Microsample ELISA Kit

CAT: 0013-GTR18873540-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873540-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse GS (Gelsolin) Microsample ELISA Kit
Product Name Alternative
GSN; AGEL; ADF; Brevin; Gelsolin Amyloidosis, Finnish Type; Actin-depolymerizing factor
UniProt
P13020
Reactivity
Mouse
Field of Research
Cancer Biology, Infectious Disease & Virology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
2.2 μg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GS in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
7.82-500 μg/mL

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