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Mouse HAase (Hyaluronidase) Microsample ELISA Kit

CAT: 0013-GTR18873478-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873478-01Size:48 Tests
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Description
Mouse HAase (Hyaluronidase) Microsample ELISA Kit
Product Name Alternative
Hyaluronic Lase; Hyaluroniclase
UniProt
Q3UZE4
Reactivity
Mouse
Field of Research
Cancer Biology, Gastroenterology & Hepatology, Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
1.16 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HAase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HAase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HAase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HAase in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
3.13-200 ng/mL

UniProtKB · Q3UZE4

Hyaluronidase

Q3UZE4_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q3UZE4
Review status
UniProtKB unreviewed (TrEMBL)
Gene
Hyal2
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.2.1.35
Processing
—
Secondary accessions
—
Protein keywords

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinGPI-anchorLipoprotein

Domain

EGF-like domainSignal

Molecular function

GlycosidaseHydrolaseReceptor

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