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Human sC5b-9 (Soluble Terminal Complement Complex C5b-9) Microsample ELISA Kit

CAT: 0013-GTR18873370-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873370-01Size:48 Tests
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Description
Human sC5b-9 (Soluble Terminal Complement Complex C5b-9) Microsample ELISA Kit
Product Name Alternative
MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
19.9 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
78.13-5000 ng/mL

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