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Human MUC1 (Mucin 1) Microsample ELISA Kit

CAT: 0013-GTR18873253-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873253-01Size:48 Tests
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Description
Human MUC1 (Mucin 1) Microsample ELISA Kit
Product Name Alternative
CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin
UniProt
P15941
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.271 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MUC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MUC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MUC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MUC1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.63-40 ng/mL

UniProtKB · P15941

Mucin-1

MUC1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P15941
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MUC1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MUC-1
EC number
—
Processing
Precursor
Secondary accessions
A5YRV1, A6ZID9, A6ZIE0, B1AVQ8, B1AVR0, B6ECA1, E7ESE5, E7EUG9, P13931, P15942, P17626, Q0VAP5, Q0VAP6, Q14128, Q14876, Q16437, Q16442, Q16615, Q6S4Y3, Q7Z547, Q7Z548, Q7Z550, Q7Z552, Q9BXA4, Q9UE75, Q9UE76, Q9UQL1, Q9Y4J2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Autocatalytic cleavageDisulfide bondGlycoproteinLipoproteinPalmitatePhosphoprotein

Cellular component

Cell membraneCytoplasmMembraneNucleusSecreted

Domain

RepeatSignalTransmembraneTransmembrane helix

Disease

Tumor suppressor

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