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Mouse RETN (Resistin) Microsample ELISA Kit

CAT: 0013-GTR18873250-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873250-01Size:48 Tests
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Description
Mouse RETN (Resistin) Microsample ELISA Kit
Product Name Alternative
ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3
UniProt
Q99P87
Reactivity
Mouse
Field of Research
Cardiovascular Research, Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.29 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RETN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RETN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RETN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RETN in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.78-50 ng/mL

UniProtKB · Q99P87

Resistin

RETN_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q99P87
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Retn
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Disease

Diabetes mellitusObesity

PTM

Disulfide bond

Molecular function

Hormone

Cellular component

Secreted

Domain

Signal

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