Products for Research Use Only

Human PLA1 (Phospholipase A1) Microsample ELISA Kit

CAT: 0013-GTR18873144-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873144-01Size:48 Tests
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Description
Human PLA1 (Phospholipase A1) Microsample ELISA Kit
Product Name Alternative
PLA1A; ps-PLA1; NMD; Phosphatidylserine-Specific Phospholipase A1-Alpha
UniProt
Q53H76
Reactivity
Human
Field of Research
Cardiovascular Research, Gastroenterology & Hepatology, Metabolism Research, Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.059 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLA1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · Q53H76

Phospholipase A1 member A

PLA1A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q53H76
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PLA1A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.1.1.111
Processing
Precursor
Secondary accessions
B2R8V2, B4DXA2, O95991, Q86WX6, Q9UPD2
Protein keywords

Coding sequence diversity

Alternative splicing

PTM

Disulfide bondGlycoprotein

Molecular function

Hydrolase

Biological process

Lipid degradationLipid metabolism

Technical term

Proteomics identificationReference proteome

Cellular component

Secreted

Domain

Signal