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Human GZMK (Granzyme K) Microsample ELISA Kit

CAT: 0013-GTR18873058-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873058-01Size:48 Tests
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Description
Human GZMK (Granzyme K) Microsample ELISA Kit
Product Name Alternative
GZM-K; TRYP2; PRSS; Serine Protease, Granzyme 3; Tryptase II; Fragmentin-3; Granzyme-3; NK-tryptase-2
UniProt
P49863
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.112 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GZMK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GZMK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GZMK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GZMK in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · P49863

Granzyme K

GRAK_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P49863
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GZMK
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
GrK
EC number
3.4.21.-
Processing
Precursor
Secondary accessions
B2R563
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondZymogen

Molecular function

HydrolaseProteaseSerine protease

Biological process

ImmunityInflammatory responseInnate immunity

Cellular component

Secreted

Domain

Signal

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