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Mouse TGFa (Transforming Growth Factor Alpha) Microsample ELISA Kit

CAT: 0013-GTR18872942-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872942-01Size:48 Tests
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Description
Mouse TGFa (Transforming Growth Factor Alpha) Microsample ELISA Kit
Product Name Alternative
TGF Alpha; ETGF; TGF-A; Protransforming Growth Factor alpha; EGF-Like TGF
UniProt
P48030
Reactivity
Mouse
Field of Research
Cancer Biology; Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
3.5 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TGFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TGFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGFa in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
7.82-500 pg/mL

UniProtKB · P48030

Protransforming growth factor alpha

TGFA_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P48030
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Tgfa
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Domain

EGF-like domainSignalTransmembraneTransmembrane helix

Molecular function

Growth factorMitogen

Technical term

Reference proteome

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