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Mouse ANG (Angiogenin) Microsample ELISA Kit

CAT: 0013-GTR18872940-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872940-01Size:48 Tests
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Description
Mouse ANG (Angiogenin) Microsample ELISA Kit
Product Name Alternative
RNASE5; Ribonuclease, RNase A Family 5
UniProt
P21570
Reactivity
Mouse
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
0.127 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ANG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ANG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ANG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ANG in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · P21570

Angiogenin

ANGI_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P21570
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ang
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.1.27.-
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Biological process

AngiogenesisDifferentiationStress response

Cellular component

CytoplasmNucleusSecreted

Molecular function

Developmental proteinDNA-bindingEndonucleaseHydrolaseNucleaseProtein synthesis inhibitor

PTM

Disulfide bondPyrrolidone carboxylic acid

Domain

Signal

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