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Human MyoD (Myogenic Differentiation) Microsample ELISA Kit

CAT: 0013-GTR18872744-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872744-01Size:48 Tests
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Description
Human MyoD (Myogenic Differentiation) Microsample ELISA Kit
Product Name Alternative
MYOD1; PUM; MYF3; bHLHc1; Myoblast Determination Protein 1; Myogenic Factor 3; Class C basic helix-loop-helix protein 1
UniProt
P15172
Reactivity
Human
Field of Research
Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.061 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MyoD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MyoD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MyoD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MyoD in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P15172

Myoblast determination protein 1

MYOD1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P15172
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MYOD1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
O75321
Protein keywords

PTM

AcetylationMethylationPhosphoproteinUbl conjugation

Molecular function

ActivatorDevelopmental proteinDNA-binding

Biological process

DifferentiationMyogenesisTranscriptionTranscription regulation

Disease

Disease variant

Cellular component

Nucleus

Technical term

Proteomics identificationReference proteome

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