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Human UCP2 (Uncoupling Protein 2, Mitochondrial) Microsample ELISA Kit

CAT: 0013-GTR18872728-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872728-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human UCP2 (Uncoupling Protein 2, Mitochondrial) Microsample ELISA Kit
Product Name Alternative
SLC25A8; UCPH; Solute carrier family 25 member 8
UniProt
P55851
Reactivity
Human
Field of Research
Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.061 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UCP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UCP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UCP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UCP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P55851

Dicarboxylate carrier SLC25A8

UCP2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P55851
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
UCP2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q4PJH8, Q53HM3
Protein keywords

Cellular component

MembraneMitochondrionMitochondrion inner membrane

Technical term

Proteomics identificationReference proteome

Domain

RepeatTransmembraneTransmembrane helix

Biological process

Transport

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