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Mouse CRT (Calreticulin) Microsample ELISA Kit

CAT: 0013-GTR18872703-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872703-01Size:48 Tests
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Description
Mouse CRT (Calreticulin) Microsample ELISA Kit
Product Name Alternative
CALR; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60
UniProt
P14211
Reactivity
Mouse
Field of Research
Metabolism Research, Musculoskeletal & Connective Tissue Research, Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.063 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CRT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CRT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CRT in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P14211

Calreticulin

CALR_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P14211
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Calr
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q3TVD2
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationDisulfide bondHydroxylation

Ligand

CalciumLectinMetal-bindingZinc

Molecular function

Chaperone

Cellular component

CytoplasmCytoplasmic vesicleEndoplasmic reticulumExtracellular matrixLysosomeSarcoplasmic reticulumSecreted

Domain

RepeatSignal

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