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Human EFNB2 (Ephrin B2) Microsample ELISA Kit

CAT: 0013-GTR18872644-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872644-01Size:48 Tests
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Description
Human EFNB2 (Ephrin B2) Microsample ELISA Kit
Product Name Alternative
EPLG5; HTKL; Htk-L; LERK5; HTK Ligand; Ligand Of Eph-Related Kinase 5; Eph-Related Receptor Tyrosine Kinase Ligand 5
UniProt
P52799
Reactivity
Human
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.06 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EFNB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EFNB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EFNB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EFNB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P52799

Ephrin-B2

EFNB2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P52799
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EFNB2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q5JV56
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

AngiogenesisDifferentiationHost-virus interactionNeurogenesis

Cellular component

Cell junctionCell membraneMembrane

Molecular function

Developmental proteinHost cell receptor for virus entryReceptor

PTM

Disulfide bondGlycoproteinMethylationPhosphoprotein

Domain

SignalTransmembraneTransmembrane helix

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