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Human EPX (Eosinophil Peroxidase) Microsample ELISA Kit

CAT: 0013-GTR18872616-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872616-01Size:48 Tests
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Description
Human EPX (Eosinophil Peroxidase) Microsample ELISA Kit
Product Name Alternative
EPO; EPP; EPX-PEN; EPER
UniProt
P11678
Reactivity
Human
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
1.1 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EPX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EPX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EPX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EPX in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
3.13-200 ng/mL

UniProtKB · P11678

Eosinophil peroxidase

PERE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P11678
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EPX
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
EPO
EC number
1.11.1.7
Processing
Precursor
Secondary accessions
Q4TVP3
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Ligand

CalciumHemeIronMetal-binding

Disease

Disease variant

PTM

Disulfide bondGlycoproteinNitration

Biological process

Hydrogen peroxide

Molecular function

OxidoreductasePeroxidase

Domain

Signal

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