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Human KERA (Keratocan) Microsample ELISA Kit

CAT: 0013-GTR18872611-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872611-01Size:48 Tests
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Description
Human KERA (Keratocan) Microsample ELISA Kit
Product Name Alternative
CNA2; SLRR2B; Keratocan Proteoglycan; Keratan sulfate proteoglycan keratocan
UniProt
O60938
Reactivity
Human
Field of Research
Disease Biomarkers
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.136 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KERA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KERA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KERA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KERA in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · O60938

Keratocan

KERA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O60938
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KERA
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
KTN
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Disease

Disease variant

PTM

Disulfide bondGlycoproteinProteoglycan

Cellular component

Extracellular matrixSecreted

Domain

Leucine-rich repeatRepeatSignal

Technical term

Proteomics identificationReference proteome

Biological process

Sensory transductionVision

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