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Human EFNA4 (Ephrin A4) Microsample ELISA Kit

CAT: 0013-GTR18872584-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872584-01Size:48 Tests
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Description
Human EFNA4 (Ephrin A4) Microsample ELISA Kit
Product Name Alternative
EFL4; EPLG4; LERK4; EPH-related receptor tyrosine kinase ligand 4
UniProt
P52798
Reactivity
Human
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.125 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EFNA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EFNA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EFNA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EFNA4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · P52798

Ephrin-A4

EFNA4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P52798
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EFNA4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
C9JHJ8, G3XAK2, O95457, Q5SR71, Q6FI57
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondGlycoproteinGPI-anchorLipoprotein

Domain

Signal

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